Ebtekar’s adviser, Fariba Ibtihaj,said the vice president tested positive for COVID-19 on Wednesday.
“Everyone in the vice president’s team was also tested and the results will be available on Saturday,” she said.
She is the VP for women and family affairs. Her symptoms are said to be mild and she has not been admitted to hospital.
Ms Ebtekar is also known as the English-language spokeswoman “Mary” for the 1979 hostage-takers who seized the US embassy in Tehran and sparked a 444-day diplomatic crisis.
On Tuesday, Iran’s Deputy Health Minister Iraj Harirchi and another lawmaker, Mahmoud Sadeghi, tested positive for the coronavirus.
The number of infected people in the country reached 245 on Thursday and 26 deaths have been confirmed.